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Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in Drosophila
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神经科学
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JoVE 杂志 神经科学
Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in Drosophila

Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in Drosophila

DOI:
10.3791/57701-v

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06:49 min

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June 02, 2018

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1Division of Biological Science, Graduate School of Science,Nagoya University, 2Department of Molecular Genetics, Institute of Life Science,Kurume University

Chapters

  • 00:04Title
  • 00:31Preparation of Larval Brain Explants
  • 02:50Acquisition of Ca2+ Fluorescence Images
  • 04:00Data Analysis
  • 05:03Results: Calcium Imaging of the Larval Brain Explant
  • 05:53Conclusion

Summary

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This paper describes a protocol for ex vivo calcium imaging of the Drosophila brain. In this method, natural or synthetic compounds can be applied to the buffer to test their ability to activate particular neurons in the brain.

Tags

Ex Vivo Calcium ImagingDrosophilaEndocrine SignalingBrain Responses神经科学Imaging ChamberGCaMPFluorescence Imaging

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