/

/

用双光子激光消融神经种群及其对斑马鱼幼虫钙成像和行为记录的评价

JoVE 杂志
神经科学

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志神经科学

Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

Please note that all translations are automatically generated. Click here for the English version.

用双光子激光消融神经种群及其对斑马鱼幼虫钙成像和行为记录的评价

DOI:

10.3791/57485-v

•

10:29 min

•

June 02, 2018

•

Akira Muto, Koichi Kawakami

¹Division of Molecular and Developmental Biology, National Institute of Genetics, Department of Genetics,SOKENDAI (The Graduate University for Advanced Studies)

Chapters

00:04Title
00:51Ablation of a Subpopulation of Neurons Using a Two-photon Laser Microscope
04:47Calcium imaging to Record Prey-Evoked Neuronal Activity in the Pretectum-Ablated Zebrafish Larvae
06:38Assessment of Behavioral Consequences Following Laser Ablation
07:30Results: Assessment of Laser Ablated Pretectal Neurons with Calcium Imaging
09:05Conclusion

Summary

自动翻译

在这里, 我们提出一个协议, 以消融基因标记亚群的神经元由双光子激光从斑马鱼幼虫。

Tags

AI Writer AI Copywriting AI Writing Assistant Auto Content Generator Article Writer Text Generator AI Writer Use Cases

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

在斑马鱼幼虫的高通量自动化的行为分析

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

双光子<em>在体内</em>树突棘中的鼠标皮质用稀疏的头颅成像准备

对于电生理和钙成像急性神经组织的长时间培养

脑切片神经元树突的双光子钙成像

在体内小鼠劣质橄榄中的钙成像

体内使用大颅窗的小鼠宽场和双光子钙成像

使用双光子显微镜对闭合颅骨创伤性脑损伤和颅窗小鼠荧光蛋白表达进行活体成像

成年斑马鱼前脑活动的双光子钙成像

在单个水箱中使用一系列行为测试对成年 Danio rerio 进行神经毒性评估

Read Article