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September 14, 2018
DOI:
1Department of Dermatology, Program in Epithelial Biology,Stanford University School of Medicine, 2Program in Cancer Biology,Stanford University School of Medicine, 3Canary Center for Cancer Early Detection, Department of Radiology,Stanford University School of Medicine, 4Department of Biology,Bridgewater State University, 5System Biosciences, 6Veterans Affairs Healthcare System
Summary
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染色质循环在基因调控中起着重要作用;然而, 没有技术上的进步, 允许选择性和可逆的修饰染色质循环。在这里, 我们描述了一个强大的体系, 染色质循环重组使用 CRISPR-dCas9 (CLOuD9), 证明有选择和可逆调节基因表达在靶点。
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Morgan, S. L., Chang, E. Y., Mariano, N. C., Bermudez, A., Arruda, N. L., Wu, F., Luo, Y., Shankar, G., Huynh, S. K., Huang, C., Pitteri, S. J., Wang, K. C. CRISPR-Mediated Reorganization of Chromatin Loop Structure. J. Vis. Exp. (139), e57457, doi:10.3791/57457 (2018).
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