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Assembly and Purification of Prototype Foamy Virus Intasomes
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Assembly and Purification of Prototype Foamy Virus Intasomes

Assembly and Purification of Prototype Foamy Virus Intasomes

DOI:
10.3791/57453-v

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10:20 min

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March 19, 2018

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, ,
1Department of Cancer Biology and Genetics,The Ohio State University College of Medicine

Chapters

  • 00:04Title
  • 00:40Annealing of vDNA
  • 02:35Intasome Assembly
  • 04:12Intasome Solubilization and Purification
  • 06:23Integration Strand Transfer Assay, Fraction Selection, and Storage
  • 08:29Results: Isolation and Evaluation of PFV Intasomes
  • 09:45Conclusion

Summary

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自动翻译

Recombinant retroviral integrase and DNA oligomers mimicking viral DNA ends can form an enzymatically active complex known as an intasome. Intasomes may be used for biochemical, structural, and kinetic studies. This protocol details how to assemble and purify prototype foamy virus intasomes.

Tags

Keywords: Foamy Virus IntasomesIntegration ComplexesViral DNAPFV IntegraseDialysisAnnealingUltracentrifugal FiltrationBuffer ExchangeUV Absorbance

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