需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
8,911 Views
•
14:55 min
•
June 24, 2018
DOI:
1Laboratory of Lipid Biochemistry, Nencki Institute of Experimental Biology,Polish Academy of Sciences, 2Université de Lyon, 3Université Lyon 1, 4L’insitut National des Sciences Appliquées (INSA) de Lyon, 5Ecole Supérieure de Chimie Physique Electronique (CPE) Lyon, 6Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR),L’institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS)
Summary
Automatically generated
We present a protocol to compare the state of minerals in vesicles released by two human bone cell lines: hFOB 1.19 and Saos-2. Their mineralization profiles were analyzed by Alizarin Red-S (AR-S) staining, ultraviolet (UV) light visualization, transmission electron microscopy (TEM) imaging and energy dispersive X-ray microanalysis (EDX).
Read Article
Cite this Article
Bozycki, L., Komiazyk, M., Mebarek, S., Buchet, R., Pikula, S., Strzelecka-Kiliszek, A. Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis. J. Vis. Exp. (136), e57423, doi:10.3791/57423 (2018).
Copy