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Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

DOI:
10.3791/57421-v

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08:26 min

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June 14, 2018

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, , , , ,
1Immunobiology of Dendritic Cells,Institut Pasteur, 2INSERM U1223, 3Laboratory of Neurogenetics and Neuroinflammation, INSERM UMR1163,Institut Imagine, 4MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine,University of Edinburgh, 5Manchester Centre for Genomic Medicine,University of Manchester

Chapters

  • 00:05Title
  • 01:03Paramagnetic Bead Preparation, Activation, and Antibody-conjugation
  • 03:26Bead Blocking and Final Clean-up
  • 04:50Assay Optimization
  • 07:06Results: Application of an Optimized IFN-α Assay
  • 07:40Conclusion

Summary

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Here we present a protocol to describe the development and validation of a single molecule array digital ELISA assay, which enables the ultra-sensitive detection of all IFN-α subtypes in human samples.

Tags

Single Molecule ArrayDigital ELISAInterferon-γUltrasensitiveBiomarker DiscoveryAutoimmune Polyendocrine Syndrome Type 1Paramagnetic BeadsEDCAntibody Conjugation

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