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Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay
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发育生物学
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JoVE 杂志 发育生物学
Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

DOI:
10.3791/57309-v

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06:21 min

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February 16, 2018

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, , ,
1Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill, 2Lineberger Comprehensive Cancer Center,University of North Carolina at Chapel Hill, 3Department of Medicine,University of North Carolina at Chapel Hill

Chapters

  • 00:04Title
  • 00:41Endothelial Cell Microcarrier Bead Coating
  • 02:31Gel Implantation
  • 04:57Results: Representative Microcarrier Bead-coating
  • 05:30Conclusion

Summary

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This protocol presents a novel in vitro bead assay that more appropriately models the process of in vivo sprouting angiogenesis by incorporating pericytes. This modification enables the bead assay to more faithfully recapitulate the heterotypic cellular interactions between endothelial cells and mural cells that are critical for angiogenesis.

Tags

PericytesEndothelial CellsAngiogenesisSprouting AssayHeterotypic Cellular InteractionsMicrocarrier BeadsCell CultureProliferationMigrationAnastomosis

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