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En gravende/Tunneling Assay til påvisning af hypoxi i Drosophila melanogaster larver
JoVE 杂志
行为学
This content is Free Access.
JoVE 杂志 行为学
A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster Larvae
DOI:

09:04 min

March 27, 2018

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Chapters

  • 00:04Title
  • 01:03Preparation of the Larvae and Assay Plates
  • 02:52Conducting the Assay
  • 04:24Preparing Assay Plates for Imaging
  • 07:06Results: Assessing RNAi Suppression of uninflatable
  • 08:32Conclusion

Summary

自动翻译

Protokollen beskriver en simpel analyse for at identificere Drosophila melanogaster larver, der oplever hypoxi under normale atmosfæriske ilt niveauer. Denne protokol tillader hypoksiske larver kan skelnes fra andre mutanter, der viser overlappende fænotyper som træghed eller langsom vækst.

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