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人类成人皮肤成纤维细胞诱导神经元高产培养的简单生成
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts
DOI:

09:07 min

February 05, 2018

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Chapters

  • 00:04Title
  • 01:02Preparation of Skin Fibroblasts for Reprogramming
  • 01:43Plating for Reprogramming (Day 1)
  • 03:22Viral Transduction (Day 0)
  • 04:12Maintenance of the Converting Cells
  • 04:58FACS
  • 07:33Results: Generation of Induced Neurons from Adult Human Dermal Fibroblasts
  • 08:15Conclusion

Summary

自动翻译

直接的神经元重编程产生的神经元保持起始体细胞的年龄。在这里, 我们描述了一个单一的矢量的方法, 产生诱导神经元从真皮成纤维细胞获得从成人捐赠人。

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