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A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans

A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans

DOI:
10.3791/56892-v

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08:49 min

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March 20, 2018

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, , ,
1The Buck Institute for Research on Aging, 2The Scripps Research Institute Center on Aging

Chapters

  • 00:04Title
  • 00:30Nematode Growth Medium (NGM) Plates and C. elegans Mass Culture Preparation
  • 03:24Hatch in Buffer to Obtain Synchronized Culture of Arrested Larva
  • 05:01Setup and Chemical Treatment of 96-well Culture/Assay Plates
  • 06:42Results: Representative Compound Screening and Re-testing
  • 08:11Conclusion

Summary

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Here we describe a simple protocol for rapidly producing hundreds of nematode growth media agar, 96-well culture plates with consistent numbers of Caenorhhabditis elegans per well. These cultures are useful for the phenotypic screening of whole organisms. We focus here on using these cultures to screen chemicals for pro-longevity effects.

Tags

Keywords: C. ElegansHigh-throughput ScreeningChemical CompoundsLifespanNematode Growth MediumE. Coli96-well PlateMass CultureGravid AdultsHypochlorite SolutionEgg Synchronization

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