需要订阅 JoVE 才能查看此. 登录或开始免费试用。
A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells
10,120 Views
•
08:45 min
•
August 22, 2018
DOI:
1Division of Experimental Hematology,Cincinnati Children’s Hospital Medical Center, 2Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute,Perelman School of Medicine, University of Pennsylvania, 3Department of Obstetrics and Gynecology, Southwest Hospital,Third Military Medical University
Summary
Automatically generated
A standard Western blotting protocol was optimized for analyzing as few as 500 hematopoietic stem or progenitor cells. Optimization involves careful handling of the cell sample, limiting transfers between tubes, and directly lysing the cells in Laemmli sample buffer.
Read Article
Cite this Article
Cai, X., Zheng, Y., Speck, N. A. A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells. J. Vis. Exp. (138), e56855, doi:10.3791/56855 (2018).
Copy