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Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate

Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate

DOI:
10.3791/56722-v

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10:05 min

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January 17, 2018

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,
1Research & Development, Immco Diagnostics,A Trinity Biotech Company

Chapters

  • 00:05Title
  • 01:01Sample and Substrate Preparation
  • 02:04Addition of Controls and Samples
  • 03:37Addition of Fluorescent Conjugate and Analysis of Results
  • 06:32Results: Classic Disease Associated ANA and DFS70 Patterns on Conventional Hep-2 and Novel Hep-2 DFS70 Knockout Substrates
  • 08:20Conclusion

Summary

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自动翻译

DFS70 autoantibodies mimic common disease-associated antinuclear antibody patterns making accurate interpretation challenging when using conventional HEp-2 substrates. The protocol describes advantages of novel engineered HEp-2 substrate over conventional HEp-2 in ANA screening and distinguishing DFS70 patterns with high confidence in both monospecific and mixed ANA positive cases.

Tags

ANA ScreeningHEp-2 ELITE/DFS70 Knockout SubstrateMonospecific DFS70 PatternMixed DFS70 PatternIndirect Immunofluorescence AssayAntinuclear AutoantibodiesHomogeneous Speckled PatternQuality ControlSubstrate Slide PreparationSample IncubationWell Cross-contaminationAutoimmune Disease

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