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使用细菌共轭的 Enterobacterial 菌株 (如大肠杆菌或志贺菌福氏) 创建稠密的转插入库
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
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使用细菌共轭的 Enterobacterial 菌株 (如大肠杆菌或志贺菌福氏) 创建稠密的转插入库

15,348 Views

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11:36 min

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September 23, 2017

DOI:

10.3791/56216-v

DOI:
10.3791/56216-v

•

11:36 min
September 23, 2017

•

9 Views
1Institute of Natural and Mathematical Sciences,Massey University

Summary

Automatically generated

这里介绍的是一个简单的方法, 创建高密度转插入库在大肠杆菌或志贺菌福氏使用细菌共轭。该协议允许通过随机的基因组插入转在细菌中创造出数十万个独特的突变体。

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Freed, N. E. Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri. J. Vis. Exp. (127), e56216, doi:10.3791/56216 (2017).

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