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低丰度胚胎标本的染色质沉淀 (芯片) 协议
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples
DOI:

12:47 min

August 29, 2017

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Chapters

  • 00:05Title
  • 00:57Preparation of Eggs and Microdissection of Chicken Spinal Neural Tube (SNT)
  • 04:12Crosslinking Proteins to DNA
  • 05:01Lysis, Sonication, and Immunoprecipitation
  • 06:33Washing, Elution, and Reversal of Crosslinking
  • 08:57Cellular Protein and RNA Digestion
  • 10:06Results: Evaluation of the ChIP Protocol by ChIP-seq Experiments
  • 11:48Conclusion

Summary

自动翻译

在这里, 我们描述了染色质沉淀 (芯片) 和芯片序列库的准备协议, 以产生全球表剖面从丰鸡胚胎标本。

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