This manuscript describes how to distinguish different nematodes using far-field diffraction signatures. We compare the locomotion of 139 wild type and 108 "Roller" C. elegans by averaging frequencies associated with the temporal Fraunhofer diffraction signature at a single location using a continuous wave laser.
Magnes, J., Hastings, H. M., Raley-Susman, K. M., Alivisatos, C., Warner, A., Hulsey-Vincent, M. Fourier-Based Diffraction Analysis of Live Caenorhabditis elegans. J. Vis. Exp. (127), e56154, doi:10.3791/56154 (2017).