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A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
JoVE 杂志
生物化学
Author Produced
This content is Free Access.
JoVE 杂志 生物化学
A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

7,828 Views

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12:05 min

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October 01, 2017

DOI:

10.3791/55923-v

DOI:
10.3791/55923-v

•

12:05 min
October 01, 2017

•

4 Views
,
1Broad Institute,Massachusetts Institute of Technology and Harvard Medical School, 2Dept. of Biological Engineering,Massachusetts Institute of Technology

Summary

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This protocol demonstrates a simple, robust and high throughput single molecule flow-stretching assay for studying one-dimensional (1D) diffusion of molecules along DNA.

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Cite this Article

Xiong, K., Blainey, P. C. A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA. J. Vis. Exp. (128), e55923, doi:10.3791/55923 (2017).

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