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1Department of Genetics,Washington University School of Medicine, 2Graduate Program in Areas of Basic and Applied Biology (GABBA), Abel Salazar Institute of Biomedical Sciences,University of Porto, 3Instituto de Investigação e Inovação em Saúde,University of Porto, 4IPATIMUP – Instituto de Patologia e Imunologia Molecular da Universidade do Porto, 5Department of Pathology & Immunology,Washington University School of Medicine
Chapters
- 00:05Title
- 01:10Testicular Tissue Single Cell Suspension Generation
- 02:26Cell Staining and Fluorescence Activiated Cell Sorting (FACS)
- 05:33Results: Representative Testicular Cell Subset Analyses
- 07:17Conclusion
Dette værk beskriver standardiseringen af en metode til opnåelse af rensede kimcellepopulationer fra testikelvæv af forskellige pattedyrsarter. Det er en simpel protokol, der kombinerer mekanisk testis dissociation, farvning med Hoechst-33342 og propidiumiodid og FACS sortering, med brede anvendelser i sammenlignende undersøgelser af reproduktionstoksikologisk biologi.
