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Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
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JoVE 杂志 神经科学
Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

DOI:
10.3791/55862-v

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07:30 min

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September 04, 2017

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, ,
1National Institute of Neurological Disorders and Stroke, 2College of Pharmacy,Chung-ang University

Chapters

  • 00:05Title
  • 00:40Culture Stimulation
  • 01:35Fixation and Staining
  • 03:42Epoxy Embedding
  • 05:43Results: Images of Presynaptic Terminal in Stimulated Neurons with High Potassium
  • 06:49Conclusion

Summary

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自动翻译

Synaptic vesicle endocytosis is detected by light microscopy of pHluorin fused with synaptic vesicle protein and by electron microscopy of vesicle uptake.

Tags

Synaptic Vesicle EndocytosisHippocampal NeuronsPoly-d-lysineHigh Potassium StimulationGlutaraldehydeSodium Cacodylate BufferDAB SolutionOsmium TetroxideSodium Acetate BufferUranyl AcetateDehydrationElectron Microscopy

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