Journal
/
发育生物学
/
Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

9,287 Views

•

08:28 min

•

June 30, 2017

DOI:

10.3791/55818-v

DOI:
10.3791/55818-v

•

08:28 min
June 30, 2017

•

2 Views
, , ,
1Department of Large Animal Clinical Sciences,Michigan State University, 2Department of Pharmacology and Toxicology,Michigan State University

Summary

Automatically generated

Here we report a method for isolation of Adipocyte Progenitor Cell (APC) populations from Perivascular Adipose Tissue (PVAT) using Magnetic-activated Cell Sorting (MCS). This method allows for an increased isolation of APC per gram of adipose tissue when compared to Fluorescence-Activated Cell Sorting (FACS).

Explore More Videos

Adipocyte Progenitor CellsMagnetic Activated Cell SortingAdipogenesisStromal Vascular FractionCollagenase DigestionCell FiltrationErythrocyte Lysis

Read Article

Cite this Article

Thelen, K., Ayala-Lopez, N., Watts, S. W., Contreras, G. A. Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting. J. Vis. Exp. (124), e55818, doi:10.3791/55818 (2017).

Download .ris file

Copy

Share Video

.

Copy