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Tidsavhengig økning i nettverksresponsen til stimulering av neuronale cellekulturer på mikroelektrodearrayer
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

Tidsavhengig økning i nettverksresponsen til stimulering av neuronale cellekulturer på mikroelektrodearrayer

DOI:
10.3791/55726-v

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10:45 min

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May 29, 2017

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, , ,
1Krasnow Institute for Advanced Study,George Mason University, 2Neural Engineering, Bioengineering,George Mason University, 3Neural Engineering, Computer Science,George Mason University, 4Electrical and Computer Engineering,George Mason University

Chapters

  • 00:05Title
  • 01:04Embryo Removal and Brain Extraction
  • 03:19Frontal Cortext Removal
  • 04:46Cell Dissociation
  • 06:48Plating Cells and Maintaining the Cultures
  • 08:01Visual Inspections and Recording
  • 09:24Results: Trained Networks Have Significantly Altered Spike Frequencies
  • 10:19Conclusion

Summary

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自动翻译

Musneuronceller dyrket på flere elektrodearrayer viser en økning i respons etter elektrisk stimulering. Denne protokollen demonstrerer hvordan man dyrker neuroner, hvordan man registrerer aktivitet, og hvordan man etablerer en protokoll for å trene nettene for å reagere på stimuleringsmønstre.

Tags

Keywords: Microelectrode ArraysNeuronal Cell CulturesNetwork DynamicsLearning And MemoryDrug ToxicityPersonalized MedicineDissectionDissociationLamininL-15 Medium

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