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Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors

Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors

DOI:
10.3791/55627-v

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10:36 min

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June 13, 2017

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, , , ,
1Department of Medical Neurobiology, Faculty of Medicine and the Edmond and Lily Safra Center for Brain Sciences (ELSC),Hebrew University, 2Department of Physiology, Development and Neuroscience,University of Cambridge

Chapters

  • 00:05Title
  • 01:01Retina Dissection and Ommatidia Isolation
  • 05:32Whole-cell Recording
  • 08:21Results: The inaCP209 and inaDP215 Mutants Reveal Slow Respomse Termination of the Microscopic Response to Light and of the Single Quantum Bumps
  • 09:50Conclusion

Summary

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Whole-cell recordings from Drosophila melanogaster photoreceptors enable the measurement of spontaneous dark bumps, quantum bumps, macroscopic responses to light, and current-voltage relationships under various conditions. In combination with D. melanogaster genetic manipulation tools, this method enables the study of the ubiquitous inositol-lipid signaling pathway and its target, the TRP channel.

Tags

Keywords: ElectrophysiologyWhole-cell Voltage ClampDrosophilaPhotoreceptorsPhototransductionTRP ChannelsVisual TransductionDissectionCalcium-free Extracellular SolutionInverted MicroscopePerfusion SystemSuction SystemGround WireElectrophysiology Setup

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