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发育生物学
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Évaluation des cardiomyocytes types suivants Reprogrammation Transcription Factor médiée par des fibroblastes embryonnaires de souris
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发育生物学
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JoVE 杂志 发育生物学
Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts
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Évaluation des cardiomyocytes types suivants Reprogrammation Transcription Factor médiée par des fibroblastes embryonnaires de souris

7,308 Views

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09:29 min

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March 22, 2017

DOI:

10.3791/55456-v

DOI:
10.3791/55456-v

•

09:29 min
March 22, 2017

•

3 Views
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1Department of Internal Medicine- Cardiology,UT Southwestern Medical Center

Summary

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This manuscript describes a step-by-step protocol for the generation and quantification of diverse reprogrammed cardiac subtypes using a retrovirus-mediated delivery of Gata4, Hand2, Mef2c, and Tbx5.

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Fernandez-Perez, A., Munshi, N. V. Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts. J. Vis. Exp. (121), e55456, doi:10.3791/55456 (2017).

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