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Chapters
- 00:04Title
- 00:44Setup of the LC/HDMSE (High Definition MSE) Data Acquisition Method
- 03:59Setup of the Tof-MRM Method for Collision Energy Optimization and the Final HS-MRM Acquisition Method for Peptide Quantification
- 07:42Creation of a Processing Method for the Analysis of the HS-MRM Dataset
- 09:57Results: An HS-MRM Assay for Quantification of Host-cell Protein Peptides in a Monoclonal Antibody Digest
- 10:29Conclusion
Tags
Keywords:
HS-MRM AssayHost-cell ProteinsProtein BiopharmaceuticalsLiquid ChromatographyIon MobilityQTOF Mass SpectrometryPeptide QuantificationProtein CharacterizationQuantitative ProteomicsLow-abundant ProteinsPeptide SeparationGradient ElutionIon Mobility SeparationHigh-resolution Mass SpectrometryHigh-definition MSECollision EnergyLock Mass Correction
