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Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
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生物化学
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Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

14,647 Views

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11:30 min

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January 26, 2017

DOI:

10.3791/55236-v

DOI:
10.3791/55236-v

•

11:30 min
January 26, 2017

•

3 Views
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1Department of Microbiology,The University of Texas Southwestern Medical Center

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Tandem Affinity PurificationProtein ComplexesEukaryotic CellsBiochemical CharacterizationMolecular AssembliesNovel InteractorsPost-translational ModificationsSTREP Affinity PurificationPassive Lysis BufferSTREP BeadsFLAG Beads

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Ma, Z., Fung, V., D'Orso, I. Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells. J. Vis. Exp. (119), e55236, doi:10.3791/55236 (2017).

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