Journal
/
神经科学
/
Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes
JoVE 杂志
神经科学
Author Produced
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes

Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes

DOI:
10.3791/55220-v

•

12:45 min

•

August 12, 2017

•

, , , , , ,
1Neural Stem Cell Institute

Chapters

  • 00:00Title
  • 00:09Outline of Technique
  • 02:37Materials Needed and Surgical Preparations
  • 05:18Surgical Procedures: Trans-sclera Subretinal Injection
  • 10:12Results
  • 11:39Conclusion

Summary

自动翻译

自动翻译

Subretinal injection has been widely applied in preclinical studies of stem cell replacement therapy for age-related macular degeneration. In this visualized article, we describe a less risky, reproducible and precisely modified subretinal injection technique via the trans-scleral approach to deliver cells into rat eyes.

Tags

Trans-scleralSubretinal TransplantationHuman Retinal Pigment Epithelial CellsRat EyesRPE CellsDegenerative RetinopathiesAge-related Macular DegenerationAMDRetinal Pigment Epithelial Cell DegenerationStem Cell Replacement TherapySubretinal Injection Technique

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/55220?article_type=v

Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology
check_url/cn/55220?article_type=v

Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium
check_url/cn/55220?article_type=v

Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol
check_url/cn/55220?article_type=v

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
check_url/cn/55220?article_type=v

Enrichment of Bruch’s Membrane from Human Donor Eyes
check_url/cn/55220?article_type=v

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
check_url/cn/55220?article_type=v

A Protocol for Measuring Cue Reactivity in a Rat Model of Cocaine Use Disorder
check_url/cn/55220?article_type=v

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains
check_url/cn/55220?article_type=v

Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains
check_url/cn/55220?article_type=v

3D Kinematic Analysis for the Functional Evaluation in the Rat Model of Sciatic Nerve Crush Injury

Read Article