Journal
/
生物化学
/
सह-immunoprecipitation द्वारा परमाणु और cytoplasmic भागों में प्रोटीन, प्रोटीन बातचीत के दृश्य और<em> बगल में</em> निकटता Ligation परख
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
Please note that all translations are automatically generated. Click here for the English version.

सह-immunoprecipitation द्वारा परमाणु और cytoplasmic भागों में प्रोटीन, प्रोटीन बातचीत के दृश्य और<em> बगल में</em> निकटता Ligation परख

12,467 Views

•

10:05 min

•

January 16, 2017

DOI:

10.3791/55218-v

DOI:
10.3791/55218-v

•

10:05 min
January 16, 2017

•

4 Views
, ,
1University Children’s Hospital Basel (UKBB),University of Basel, 2Department of Clinical Research,University of Basel

Tags

Protein-protein InteractionCo-immunoprecipitationIn Situ Proximity Ligation AssayNuclear FractionCytoplasmic FractionCellular CompartmentsHEK293 CellsCytoplasmic ExtractionNuclear ExtractionProtein G Magnetic BeadsInput Samples

Read Article

Cite this Article

Zhu, X., Zelmer, A., Wellmann, S. Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay. J. Vis. Exp. (119), e55218, doi:10.3791/55218 (2017).

Download .ris file

Copy

Share Video

.

Copy