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Swab Sampling Verfahren zum Nachweis von humanem Norovirus auf Oberflächen
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Swab Sampling Method for the Detection of Human Norovirus on Surfaces
DOI:

10:03 min

February 06, 2017

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Chapters

  • 00:05Title
  • 01:04Swab Sampling, Virus Concentration, Viral RNA Extraction, and Purification
  • 02:36Midi Column Viral Nucleic Acid Extraction
  • 03:40Viral Nucleic Acid Concentration
  • 05:16Multiplex RT-qPCR Detection
  • 07:09Quantification of Norovirus in Swab Samples and Hemi-nested Conventional PCR Amplification
  • 08:11Results: Macrofoam-based Swab Sampling Method Effectively Detects Norovirus Outbreaks
  • 09:24Conclusion

Summary

自动翻译

A macrofoam based sampling methodology was developed and evaluated for the detection and quantification of norovirus on environmental hard surfaces.

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