Here, we describe a protocol for radiolabeling and in vivo testing of tridentate 99mTc(I) chelate-tetrazine derivatives for pre-targeting and bioorthogonal chemistry.
Bilton, H. A., Ahmad, Z., Janzen, N., Czorny, S., Valliant, J. F. Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry. J. Vis. Exp. (120), e55188, doi:10.3791/55188 (2017).