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Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light
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神经科学
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JoVE 杂志 神经科学
Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

DOI:
10.3791/55176-v

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11:36 min

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February 10, 2017

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, , , , ,
1Department of Neuroscience of Disease, Center for Transdisciplinary Research,Niigata University, 2Brain Research Institute,Niigata University, 3Image and Data Analysis Facility,German Center for Neurodegenerative Diseases (DZNE), 4Graduate School of Life Science and Technology,Tokyo Institute of Technology (Titech), 5Dendrite Differentiation,German Center for Neurodegenerative Diseases (DZNE)

Chapters

  • 00:05Title
  • 00:47Treatment of Flies and Imaging Brains
  • 02:54Image Analysis: Generating the Spots, Surface Objects, Start-points, and End-points
  • 08:04Image Analysis: Calculating the Number of Spots, Distribution of Spot Frequency and Enrichment Level of Brp-GFP
  • 09:48Results: Brp-GFP Expression in R8 Synapses of the Medulla
  • 11:02Conclusion

Summary

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Here we show how to quantify the number and spatial distribution of synaptic active zones in Drosophila melanogaster photoreceptors, highlighted with a genetically encoded molecular marker, and their modulation after prolonged exposure to light.

Tags

Synaptic ModulationDrosophila MelanogasterPhotoreceptorsProlonged Light ExposureSynaptic PlasticitySynaptic DynamicsNeuron ActivitySynaptic AnalysisBrp ExpressionR7 And R8 Photoreceptor AxonsImmunolabeling3D ImagingImage AnalysisSpot Detection

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