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嗜肺军团菌外膜囊泡：隔离及对巨噬细胞促炎潜力分析
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
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嗜肺军团菌外膜囊泡：隔离及对巨噬细胞促炎潜力分析

DOI:
10.3791/55146-v

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08:34 min

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February 22, 2017

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, , , , ,
1Institute for Lung Research, Universities of Giessen and Marburg Lung Center,Philipps-University Marburg, 2German Center for Lung Research, 3Department of Medicine, Pulmonary and Critical Care Medicine,University Medical Center Giessen and Marburg

Chapters

  • 00:05Title
  • 01:18Cultivate L. pneumophila and Prepare and Quantify L. pneumophila Outer Membrane Vesicles (OMVs)
  • 03:42Pre-treat, Infect Macrophages, and Assess Bacterial Replication with a Colony-Forming Unit (CFU) Assay
  • 05:31Results: Pro-inflammatory Response of Macrophages from L. pneumophila Infection
  • 07:04Conclusion

Summary

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自动翻译

这里，我们描述嗜肺军团菌 （ 嗜肺军团菌 ）从液体培养外膜囊泡（OMV的）的纯化。然后将这些纯化的囊泡用于巨噬细胞的治疗，以分析其促炎潜力。

Tags

Legionella PneumophilaOuter Membrane Vesicles (OMVs)MacrophagesPro-inflammatory PotentialIsolationPurificationStandardized ProtocolHost-pathogen Interaction

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