需要订阅 JoVE 才能查看此. 登录或开始免费试用。
1Center for Metabolic and Degenerative Diseases, The Brown Foundation Institute of Molecular Medicine, Programs in Human and Molecular Genetics and Neuroscience, The Graduate School of Biomedical Sciences at Houston,The University of Texas Health Science Center at Houston, 2Department of Natural Sciences,University of Houston – Downtown, 3Department of Neurobiology and Anatomy, McGovern Medical School,The University of Texas Health Science Center at Houston
Chapters
- 00:05Title
- 01:12Microsurgical Dissection of Adult Fly Heads
- 02:56Immunofluorescent Staining of the Brains
- 04:44Mounting and Imaging of Brain Tissue
- 06:02Results: Anti-TH Staining in Dissected Fly Brains Allows Quantification of DA Neurons
- 07:40Conclusion
O adulto de Drosophila cérebro é um sistema valioso para estudar circuitos neuronais, funções cerebrais superiores, e doenças complexas. Um método eficiente para dissecar tecido do cérebro inteiro do cabeça pequena mosca facilitará estudos baseados em cerebrais. Aqui nós descrevemos um de uma etapa simples protocolo, dissecação de cérebros adultos com morfologia bem preservada.
