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Utilizzare risorse CyVerse per<em> De Novo</em> Transcriptomics comparativi degli organismi non sottoposti a modellazione (non modello)
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms
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Utilizzare risorse CyVerse per<em> De Novo</em> Transcriptomics comparativi degli organismi non sottoposti a modellazione (non modello)

8,987 Views

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10:41 min

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May 09, 2017

DOI:

10.3791/55009-v

DOI:
10.3791/55009-v

•

10:41 min
May 09, 2017

•

1 Views
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1BIO5 Institute,University of Arizona, 2The School of Plant Sciences,University of Arizona, 3Genetics GIDP,University of Arizona, 4Biology Department,University of Massachusetts Amherst, 5School of Animal and Comparative Biomedical Sciences,University of Arizona, 6CyVerse,University of Arizona

Summary

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This protocol outlines a comparative de novo transcriptome assembly and annotation workflow for novice bioinformaticians. The workflow is available for free entirely through CyVerse and connected by the Data Store. Command line and graphical user interfaces are used, but all code needed is available to copy and paste.

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Cite this Article

Joyce, B. L., Haug-Baltzell, A. K., Hulvey, J. P., McCarthy, F., Devisetty, U. K., Lyons, E. Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms. J. Vis. Exp. (123), e55009, doi:10.3791/55009 (2017).

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