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Isolement et culture des adultes cellules souches neurales de la Subcallosal Zone Souris
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone
DOI:

09:24 min

December 15, 2016

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Chapters

  • 00:05Title
  • 00:49Preparation of Medium and Coating Plate
  • 02:09Isolation and Dissociation of the Adult SCZ
  • 03:38SCZ-derived aNSC Culture
  • 05:18Passaging and Differentiation of SCZ-aNSCs
  • 07:22Results: Maintenance of the SCZ-aNSCs as a Monolayer Culture
  • 08:17Conclusion

Summary

自动翻译

Establishing culture systems for the expansion of adult neural stem cells (aNSCs) allows for the examination and application of aNSCs for therapy. The subcallosal zone (SCZ) has recently been recognized as a novel neuroblast-forming region in adult mice. Here, methods for the isolation, expansion, and differentiation of SCZ-aNSCs are described.

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