Nanoparticle-based optical probes have been designed as a vehicle for detecting antigens using Raman and UV-Vis spectroscopy. Here we describe a protocol for preparing such probes for a UV-Vis/Raman spectroscopy immunoassay in such a way to incorporate future multiplexing capabilities.
Hanson, C., Israelsen, N. D., Sieverts, M., Vargis, E. Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform. J. Vis. Exp. (117), e54795, doi:10.3791/54795 (2016).