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Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons
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Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons

Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons

DOI:
10.3791/54767-v

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09:11 min

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August 23, 2016

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,
1Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center, 2Department of Surgery,Cedars-Sinai Medical Center, 3Department of Surgery, David Geffen School of Medicine,University of California, Los Angeles

Chapters

  • 00:05Title
  • 00:43Production of Zika Virus
  • 03:21Measuring Zika Virus Titer by Plaque Assay
  • 04:36Detection of Zika Virus dsRNA by Immunocytochemistry
  • 06:10Screening a Cytokine Library against Zika Virus Infection
  • 07:00Results: Zika Virus Replication and Effect of Interferons
  • 08:14Conclusion

Summary

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Zika Virus (ZIKV), an emerging pathogen, is linked to fetal developmental abnormalities and microcephaly. The establishment of an effective infectious cell culture system is crucial for studies of ZIKV replication as well as vaccine and drug development. In this study, various virological assays pertaining to ZIKV are illustrated and discussed.

Tags

Zika VirusInfectious Cell Culture SystemIn VitroProphylactic EffectInterferonsVero CellsViral InoculumViral PlaqueCell Culture SupernatantDrug DevelopmentVaccine Development

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