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Parallel Messung von Circadian Clock Genexpression und Hormon-Sekretion in primären humanen Zellkulturen
Chapters
- 00:05Title
- 00:52Continuos Long-term Circadian Bioluminescence Recording, Performed in Parallel with the Assessment of Hormone Secretion in Living Human Primary Cells
- 02:24Parallel Assessment of Circadian Bioluminescence Recording and Hormone Secretion Profiles in Synchronized Human Secretory Primary Cells
- 04:37Results: Evaluation of the Clock Regulation of Basal IL-6 Secretion by Human Myotubes
- 05:33Conclusion
Here, we describe settings to monitor in parallel circadian bioluminescence and the secretory activity of human islet cells and primary myotubes. For this, we employed lentiviral gene delivery of a luciferase core clock reporter, followed by in vitro synchronization and collection of outflow medium by continuous cell perifusion.
