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1Department of Pediatric Surgery, The Second Affiliated Hospital & Yuying Children’s Hospital,Wenzhou Medical University, 2Departments of Urology,Boston Children’s Hospital, 3Department of Surgery,Harvard Medical School, 4Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute,Guangdong General Hospital, Guangdong Academy of Medical Sciences
Chapters
- 00:05Title
- 00:45Mouse Embryo Pretreatment and Staining
- 01:43Infiltration and Embedding
- 03:28Image Acquisition
- 05:25Results: Representative High-resolution Episcopic Microscopy (HREM) e15.5 Embryonic Imaging
- 05:58Conclusion
We describe a detailed protocol using high-resolution episcopic microscopy to acquire three-dimensional (3D) images of mouse embryos. This improved protocol utilizes a modified tissue preparation method to enhance penetration of the fluorescent dye, thereby permitting morphometric analysis of both small and large-sized specimens.
