In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ

Tobias Ackels; Daniela R. Drose; Marc Spehr

doi:10.3791/54517

Journal

/

神经科学

/

在小鼠犁鼻急性组织切片定义细胞群的深入生理学分析

JoVE 杂志
神经科学

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志神经科学

In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ

Please note that all translations are automatically generated. Click here for the English version.

在小鼠犁鼻急性组织切片定义细胞群的深入生理学分析

DOI:

10.3791/54517-v

•

10:11 min

•

September 10, 2016

•

Tobias Ackels², Daniela R. Drose, Marc Spehr

¹Department of Chemosensation, Institute for Biology II,RWTH Aachen University, ²Mill Hill Laboratory,The Francis Crick Institute

Chapters

00:05Title
00:42VNO Dissection and Embedding
04:50Coronal VNO Tissue Slicing
05:48Single-cell Electrophysiological Recordings
08:03Results: Isolated Voltage-gated Na⁺ and Ca²⁺ Currents as well as Spike Discharge
09:24Conclusion

Summary

自动翻译

Here, we describe a physiological approach that allows identification and in-depth analysis of a defined population of sensory neurons in acute coronal tissue slices of the mouse vomeronasal organ using whole-cell patch-clamp recordings.