Journal
/
/
Utveckling av ett antigen-driven kolit modell för att studera Presentation av antigener genom antigenpresenterande celler till T-celler
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
DOI:

06:57 min

September 18, 2016

, , ,

Chapters

  • 00:05Title
  • 01:28Colonic Lamina propria Cell Isolation
  • 03:58Results: Host CX3CR1+ Macrophages Communicate with Donor OT-II Cells During OVA-driven Transfer Colitis
  • 06:03Conclusion

Summary

自动翻译

In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).

Related Videos

Read Article