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Chapters
- 00:05Title
- 00:50Isolation and Cryopreservation of Monocytes
- 01:58Differentiation of Monocytes into Macrophages
- 03:31Harvesting Macrophages to Initiate Experiments
- 05:30Results: Comparison of Fresh versus Cryopreserved Monocytes Used to Produce Macrophages
- 06:12Conclusion
A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.
