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从人胎盘组织源分离和间充质干扩张/基质细胞
JoVE 杂志
发育生物学
Author Produced
This content is Free Access.
JoVE 杂志 发育生物学
Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
DOI:

12:40 min

June 06, 2016

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Chapters

  • 00:00Title
  • 01:00Methodologies
  • 02:20Introduction
  • 03:12Tissues to be Harvested
  • 03:53Preparation of the Placenta and the Placental Anatomy
  • 04:40Dissection of the Decidua (D) Tissue
  • 05:40Dissection of the Chorionic Plate (CP) Tissue and Dissection of the Chorionic Villi (CV) Tissue
  • 06:36Mincing and Enzymatic Digestion of the Placental Tissue
  • 07:25Collection of Mononuclear Cells and Plating into Flasks
  • 09:33Post Isolation – Cell Growth and Expansion
  • 09:57Representative Results: Culture MSC and Assay Cells for MSC Characteristics
  • 12:07Conclusion

Summary

自动翻译

这里我们描述了从人足月胎盘的胎儿和母体组织的解剖方法,随后分离并从这些组织的间充质干细胞/基质细胞(MSC)的扩展。

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