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פנוטיפי אנליזה פונקציונלית של תאים תקינה T הופעל מבודדת מן עכברים הנגועים בנגיף לימפוציטית כרונית Choriomeningitis
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免疫与感染
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JoVE 杂志 免疫与感染
Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
DOI:

07:17 min

June 22, 2016

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Chapters

  • 00:05Title
  • 00:48Conventional (Tconv) and Regulatory (Treg) Splenic T Cell Phenotyping
  • 01:58In Vitro Suppression Assay Using CD4+CD25+ Treg and CD8+ T Cells
  • 03:16CD8+ T Cytokine Production and Cell Proliferation Analyses
  • 04:26Results: Activated Treg Cell-mediated Suppression of the CD8+ Responder T Cell Response
  • 06:16Conclusion

Summary

自动翻译

Here, we describe a protocol to analyze the phenotype of regulatory T (Treg) cells isolated from naïve and chronic lymphocytic choriomeningitis virus-infected mice. In addition, we provide a process to evaluate the suppressive activity of the Treg cells.

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