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FRET Imaging in idrogel tridimensionali
JoVE 杂志
生物学
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JoVE 杂志 生物学
FRET Imaging in Three-dimensional Hydrogels
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FRET Imaging in idrogel tridimensionali

12,673 Views

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09:47 min

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August 01, 2016

DOI:

10.3791/54135-v

DOI:
10.3791/54135-v

•

09:47 min
August 01, 2016

•

9 Views
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1Department of Oral Biology, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine,University of Pittsburgh, 2Department of Bioengineering, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine,University of Pittsburgh, 3Laerdal AS

Summary

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Förster resonance energy transfer (FRET) imaging is a powerful tool for real-time cell biology studies. Here a method for FRET imaging cells in physiologic three-dimensional (3D) hydrogel microenvironments using conventional epifluorescence microscopy is presented. An analysis for ratiometric FRET probes that yields linear ratios over the activation range is described.

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Cite this Article

Donius, A. E., Bougoin, S. V., Taboas, J. M. FRET Imaging in Three-dimensional Hydrogels. J. Vis. Exp. (114), e54135, doi:10.3791/54135 (2016).

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