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FRET成像的三维水凝胶
JoVE 杂志
生物学
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JoVE 杂志 生物学
FRET Imaging in Three-dimensional Hydrogels
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FRET成像的三维水凝胶

12,672 Views

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09:47 min

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August 01, 2016

DOI:

10.3791/54135-v

DOI:
10.3791/54135-v

•

09:47 min
August 01, 2016

•

9 Views
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1Department of Oral Biology, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine,University of Pittsburgh, 2Department of Bioengineering, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine,University of Pittsburgh, 3Laerdal AS

Summary

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荧光共振能量转移（FRET）成像是用于实时细胞生物学的研究的强有力的工具。这里提出了在使用常规荧光显微镜生理三维（3D）水凝胶的微环境FRET显像细胞的方法。被描述为比例FRET探针产生在激活范围线性比例的分析。

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Cite this Article

Donius, A. E., Bougoin, S. V., Taboas, J. M. FRET Imaging in Three-dimensional Hydrogels. J. Vis. Exp. (114), e54135, doi:10.3791/54135 (2016).

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