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完整的工作流程为组蛋白翻译后修饰的分析采用自下而上质谱法：从组蛋白提取数据分析
JoVE 杂志
生物学
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JoVE 杂志 生物学
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
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完整的工作流程为组蛋白翻译后修饰的分析采用自下而上质谱法：从组蛋白提取数据分析

27,617 Views

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11:02 min

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May 17, 2016

DOI:

10.3791/54112-v

DOI:
10.3791/54112-v

•

11:02 min
May 17, 2016

•

45 Views
, , , ,
1Epigenetics Program, Department of Biochemistry and Biophysics, Perelman School of Medicine,University of Pennsylvania

Summary

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这个协议概述了表征使用质谱（MS）组蛋白的翻译后修饰的完全集成的工作流程。该工作流包括使用纳米流液相色谱法和用于数据分析的指令的细胞培养物或组织中，组蛋白衍生和消化，MS分析组蛋白纯化。该协议被设计为内完成2 - 3天。

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Cite this Article

Sidoli, S., Bhanu, N. V., Karch, K. R., Wang, X., Garcia, B. A. Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis. J. Vis. Exp. (111), e54112, doi:10.3791/54112 (2016).

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