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1Center for Cancer Pharmacology,University of Pennsylvania, 2Center for Excellence in Environmental Toxicology,University of Pennsylvania, 3Penn SRP and Department of Systems Pharmacology and Translational Therapeutics,University of Pennsylvania, 4Division of Traumatology, Department of Surgery, Critical Care and Acute Care Surgery,University of Pennsylvania, 5A.J. Drexel Autism Institute,Drexel University
Chapters
- 00:05Title
- 00:38Preparation of Buffers and 100X Stock Solutions
- 01:35Platelet Isolation and Performing an Experiment
- 02:49Quenching and CoA Extraction
- 04:30Results: Analysis of Human Platelets to Study Mitochondrial Metabolism
- 05:21Conclusion
Her viser vi isolerede humane blodplader kan anvendes som en tilgængelig ex vivo-model til at studere metaboliske tilpasninger som reaktion på komplekset I inhibitoren rotenon. Denne fremgangsmåde anvender isotopisk sporing og relativ kvantificering ved væskekromatografi-massespektrometri og kan anvendes til en række af undersøgelsestyper.
