Journal
/
生物学
/
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
JoVE 杂志
生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物学
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

DOI:
10.3791/53827-v

•

11:02 min

•

May 27, 2016

•

, , , , , ,
1Greenebaum Cancer Center,University of Maryland School of Medicine, 2Pharmaceutical Sciences,University of Maryland School of Pharmacy, 3Baltimore VA Medical Center, 4Membrane Transport and Biopharmaceutics, School of Pharmaceutical Sciences,Kanazawa University, 5Obstetrics, Gynecology and Reproductive Science,University of Pittsburgh, 6Magee Women’s Research Institute, 7Obstetrics, Gynecology, Perinatal Research Branch (NICHD),Wayne State University School of Medicine, 8医学,University of Maryland School of Medicine, 9Pathology,University of Maryland School of Medicine, 10Pharmacology,University of Maryland School of Medicine, 11Experimental Therapeutics,University of Maryland School of Medicine

Chapters

  • 00:05Title
  • 01:04In Silico Prediction of Aternative First Exons of Bcrp1 Using BLAST Analysis of Mouse EST Database
  • 04:30Design of Reporter Constructs for Bcrp1 E1U, E1A, E1B, and E1C Promoters
  • 07:55Reporter Assay Methods
  • 09:03Results: Evaluation of Bcrp1 Alternative Promoter Function
  • 10:07Conclusion

Summary

自动翻译

自动翻译

With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter assays.

Tags

Alternative Promoter UsageTranscriptional RegulationBcrp1MRNA ExpressionReporter AssaysGene RegulationBLASTESTIn-silico MethodsSequence Alignment

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Dissection and Staining of Drosophila Larval Ovaries

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates

Read Article