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Chapters
- 00:05Title
- 00:38Bulk Analysis of Metalloprotein Standards using Inductively Coupled Plasma – Mass Spectrometry (ICP-MS)
- 02:41HPLC System Setup and Size Exclusion Chromatography Column Equilibration
- 04:00Setting Up and Running Size Exclusion – Inductively Coupled Plasma – Mass Spectrometry (SEC-ICP-MS)
- 06:41Results: Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
- 08:25Conclusion
Size exclusion chromatography hyphenated with inductively coupled plasma - mass spectrometry (ICP-MS) is a powerful tool to measure changes in the abundance of metalloproteins directly from biological samples. Here we describe a set of metalloprotein standards used to estimate molecular mass and the amount of metal associated with unknown proteins.
Tags
Quantitative Metalloproteomic AnalysisSize Exclusion ICP-MSMetalloprotein StandardsMetal Status Of A ProteinNative State Protein MeasurementCell Culture SamplesTris Buffered SalineCentrifugationProtein ConcentrationsInductively Coupled Plasma Mass SpectrometerDilution Of Metalloprotein StandardsCalibration Levels
