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Samtidig To-foton<em> In vivo</em> Imaging af synaptisk indgange og Postsynaptiske Mål i Mouse retrospenialis Cortex
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
DOI:

16:45 min

March 13, 2016

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Chapters

  • 00:00Title
  • 01:27Surgery Preparation
  • 03:37Cranial Window Surgery
  • 08:30Virus Injection
  • 10:43Cranial Window Implantation
  • 13:15Imaging
  • 15:12Results
  • 15:56Conclusions

Summary

自动翻译

This video shows the craniotomy procedure that allows chronic imaging of neurons in mouse retrosplenial cortex using in vivo two photon microscopy in Thy1-GFP transgenic line. This approach is combined with injection of mCherry-expressing adeno-associated virus into dorsal hippocampus. These techniques allow long-term monitoring of experience-dependent structural plasticity in RSC.

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