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Chapters
- 00:05Title
- 00:56Peripheral Blood Mononuclear Cell (PBMC) Isolation
- 01:57Vδ1 T Cell Isolation
- 06:09Limited Dilution Single Cell Cloning
- 07:32Results: Representative Vδ1+CD4+ γδ T Cell Expansion
- 09:12Conclusion
Here, we provide an optimized protocol for the isolation and cloning of the scarce T-cell entity of peripheral Vδ1+CD4+ T cells that is, as we showed recently, an extrathymic αβ T-cell progenitor. This technique allows to quantitatively isolate, clone and efficiently expand these cells in ex vivo culture.
